Open AccessEffects of dual deletion of glnR and mtrA on expression of nitrogen metabolism genes in Streptomyces venezuelae
Author(s) -
Zhu Yanping,
Wang Jiao,
Su Wenya,
Lu Ting,
Li Aiying,
Pang Xiuhua
Publication year - 2022
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.14016
Subject(s) - gene , mutant , biology , secondary metabolism , transcription factor , genetics , microbiology and biotechnology , biosynthesis
Summary GlnR activates nitrogen metabolism genes under nitrogen‐limited conditions, whereas MtrA represses these genes under nutrient‐rich conditions in Streptomyces . In this study, we compared the transcription patterns of nitrogen metabolism genes in a double deletion mutant (Δ mtrA ‐ glnR ) lacking both mtrA and glnR and in mutants lacking either mtrA (Δ mtrA ) or glnR (Δ glnR ). The nitrogen metabolism genes were expressed similarly in Δ mtrA ‐ glnR and Δ glnR under both nitrogen‐limited and nutrient‐rich conditions, with patterns distinctly different from that of Δ mtrA , suggesting a decisive role for GlnR in the control of nitrogen metabolism genes and further suggesting that regulation of these genes by MtrA is GlnR‐dependent. MtrA and GlnR utilize the same binding sites upstream of nitrogen metabolism genes, and we showed stronger in vivo binding of MtrA to these sites under nutrient‐rich conditions and of GlnR under nitrogen‐limited conditions, consistent with the higher levels of MtrA or GlnR under those respective conditions. In addition, we showed that both mtrA and glnR are self‐regulated. Our study provides new insights into the regulation of nitrogen metabolism genes in Streptomyces .