Open AccessProduction of xylitol by Saccharomyces cerevisiae using waste xylose mother liquor and corncob residues
Author(s) -
He Yao,
Li Hongxing,
Chen Liyuan,
Zheng Liyuan,
Ye Chunhui,
Hou Jin,
Bao Xiaoming,
Liu Weifeng,
Shen Yu
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13881
Subject(s) - corncob , xylitol , xylose , fermentation , chemistry , cellulase , biochemistry , pentose , food science , xylose metabolism , hydrolysis , organic chemistry , raw material
Summary Exorbitant outputs of waste xylose mother liquor (WXML) and corncob residue from commercial‐scale production of xylitol create environmental problems. To reduce the wastes, a Saccharomyces cerevisiae strain tolerant to WXML was conferred with abilities to express the genes of xylose reductase, a xylose‐specific transporter and enzymes of the pentose phosphate pathway. This strain showed a high capacity to produce xylitol from xylose in WXML with glucose as a co‐substrate. Additionally, a simultaneous saccharification and fermentation (SSF) process was designed to use corncob residues and cellulase instead of directly adding glucose as a co‐substrate. Xylitol titer and the productivity were, respectively, 91.0 g l ‐1 and 1.26 ± 0.01 g l ‐1 h ‐1 using 20% WXML, 55 g DCW l ‐1 delignified corncob residues and 11.8 FPU g cellulose ‐1 cellulase at 35° during fermentation. This work demonstrates the promising strategy of SSF to exploit waste products to xylitol fermentation process.