English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Summary  The lambda phage Red proteins Redα/Redβ/Redγ and Rac prophage RecE/RecT proteins are powerful tools for precise and efficient genetic manipulation but have been limited to only a few prokaryotes. Here, we report the development and application of a new recombineering system for  Burkholderia glumae  and  Burkholderia plantarii  based on three Rac bacteriophage RecET‐like operons, RecEThe BDU8 , RecETh TJI49  and RecETh1h2e YI23 , which were obtained from three different  Burkholderia  species. Recombineering experiments indicated that RecETh TJI49  and RecETh1h2e YI23  showed higher recombination efficiency compared to RecEThe BDU8  in  Burkholderia glumae  PG1. Furthermore, all of the proteins currently categorized as hypothetical proteins in RecETh1h2e YI23,  RecETh TJI49  and RecEThe BDU8  may have a positive effect on recombination in  B. glumae  PG1 except for the h2 protein in RecETh1h2e YI23 . Additionally, RecET YI23  combined with exonuclease inhibitors Pluγ or Redγ exhibited equivalent recombination efficiency compared to Redγβα in  Escherichia coli , providing potential opportunity of recombineering in other Gram‐negative bacteria for its loose host specificity. Using recombinase‐assisted  in situ  insertion of promoters, we successfully activated three cryptic non‐ribosomal peptide synthetase biosynthetic gene clusters in  Burkholderia  strains, resulting in the generation of a series of lipopeptides that were further purified and characterized. Compound  7  exhibited significant potential anti‐inflammatory activity by inhibiting lipopolysaccharide‐stimulated nitric oxide production in RAW 264.7 macrophages. This recombineering system may greatly enhance functional genome research and the mining of novel natural products in the other species of the genus  Burkholderia  after optimization of a protocol.

Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii