Open AccessEnhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering
Author(s) -
Liu Qingshu,
Zhang Liang,
Wang Yunsheng,
Zhang Cuiyang,
Liu Tianbo,
Duan Caichen,
Bian Xiaoying,
Guo Zhaohui,
Long Qingshan,
Tang Ying,
Du Jie,
Liu Aiyu,
Dai Liangying,
Li Dingjun,
Chen Wu
Publication year - 2022
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13825
Subject(s) - promoter , homologous recombination , biology , yield (engineering) , gene cluster , recombinant dna , gene , antimicrobial , metabolic engineering , microbiology and biotechnology , biochemistry , gene expression , materials science , metallurgy
Summary Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and immunosuppressive activities. However, the yield of edeines in wild‐type (WT) Brevibacillus is extremely low, and chemical synthesis of edeines is a time‐consuming process. Genetic engineering has proven to be an effective approach to produce antibiotics with high yield. In this study, the ede ine b iosynthetic g ene c luster ( ede BGC), which is involved in edeine production, was identified and characterized in Brevibacillus brevis X23. To improve edeine production in B. brevis X23, the ede BGC promoter was replaced with six different promoters, P mwp , P spc , P xylA , P shuttle‐09 , P grac or P 43 , through double‐crossover homologous recombination. The new promoters significantly increased the expression of the ede BGC as well as edeine production by 2.9 ± 0.4 to 20.5 ± 1.2‐fold and 3.6 ± 0.1to 8.7 ± 0.7‐fold respectively. The highest yield of edeines (83.6 mg l −1 ) was obtained in B. brevis X23 with the P mwp promoter. This study provides a practical approach for producing high yields of edeines in B . brevis .