Open AccessLabel‐free proteomic dissection on dptP ‐deletion mutant uncovers dptP involvement in strain growth and daptomycin tolerance of Streptomyces roseosporus
Author(s) -
Zhang Dan,
Wang Xixi,
Ye Yang,
He Yu,
He Fuqiang,
Tian Yongqiang,
Luo Yunzi,
Liang Shufang
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13736
Subject(s) - daptomycin , biology , gene , streptomyces , gene cluster , mutant , secondary metabolism , transcription (linguistics) , cell growth , gene expression , cell division , microbiology and biotechnology , biochemistry , genetics , cell , biosynthesis , bacteria , vancomycin , staphylococcus aureus , linguistics , philosophy
The combination of label‐free quantification proteomic dissections with loss‐ and gain‐of‐function experiments was carried out to decipher dptP ‐involved functions. DptP gene contributes to Streptomcyes primary growth under elevated temperature and DAP treatment, whereas it plays negative roles on metabolism of secondary metabolites and transcription of DAP biosynthetic genes. The strategies used in this study provide references for the follow‐up study on other relative genes of dpt gene cluster and metabolic pathway optimization for S. roseosporus .