Open AccessPolyploid engineering by increasing mutant gene dosage in yeasts
Author(s) -
Fukuda Nobuo,
Honda Shinya,
Fujiwara Maki,
Yoshimura Yuko,
Nakamura Tsutomu
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13731
Subject(s) - saccharomyces cerevisiae , mutant , biology , yeast , gene dosage , ploidy , fermentation , gene , polyploid , ethyl methanesulfonate , genome , genetics , biochemistry , gene expression
FAS2 encoding α subunit of fatty acid synthetase is one of essential genes in S. cerevisiae , and the FAS2 ‐ G1250S mutation decreases carbon chain elongation activity during fatty acid synthesis, resulting in high productivity of ethyl caproate. Evaluation for mutant gene dosage using polyploids with isogenic background revealed the trade‐off relationship between ethyl caproate productivity and cell growth rate. We further demonstrated the possibility to increase mutant gene dosage via loss of heterozygosity in diploid and tetraploid strains.