Open AccessBacteriophage‐mediated interference of the c‐di‐GMP signalling pathway in Pseudomonas aeruginosa
Author(s) -
De Smet Jeroen,
Wagemans Jeroen,
Hendrix Hanne,
Staes Ines,
Visnapuu Annegrete,
Horemans Benjamin,
Aertsen Abram,
Lavigne Rob
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13728
Subject(s) - biofilm , pseudomonas aeruginosa , bacteriophage , pyocyanin , motility , quorum sensing , lytic cycle , microbiology and biotechnology , gtp' , biology , chemistry , intracellular , lysogenic cycle , bacteria , biochemistry , gene , genetics , escherichia coli , enzyme , virus
C‐di‐GMP is a key signaling molecule which impacts bacterial motility and biofilm formation and is formed by the condensation of two GTP molecules by a diguanylate cyclase. We here describe the identification and characterization of a family of bacteriophage‐encoded peptides that bind the Pseudomonas aeruginosa diguanylate cyclase YfiN and thereby impact c‐di‐GMP signaling. This intracellular signaling interference strategy by a lytic phage constitutes an unexplored phage‐based mechanism of metabolic regulation and could potentially serve as inspiration for the development of molecules that interfere with biofilm formation in P . aeruginosa and other pathogens.