Open AccessNutrient conditions determine the localization of Bacillus thuringiensis Vip3Aa protein in the mother cell compartment
Author(s) -
Wang Zeyu,
Gan Chunxia,
Wang Jian,
Bravo Alejandra,
Soberón Mario,
Yang Qing,
Zhang Jie
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13719
Subject(s) - bacillus thuringiensis , secretion , spodoptera , biology , cell culture , toxicity , bacteria , microbiology and biotechnology , secretory protein , cell , biochemistry , chemistry , recombinant dna , gene , genetics , organic chemistry
Vip3Aa proteins produced by three different Bt strains, including an industrial strain, were retained in the mother cell compartment. However in rich medium, a fraction of Vip3Aa was secreted, suggesting that Vip3Aa secretion is nutrient‐dependent. Hence, we speculate that the accumulation of Vip3Aa protein in the mother cell compartment under sporulation conditions could still be used as an efficient strategy for industrial production in commercial Bt strains.