Open AccessLoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
Author(s) -
Shaw Daniel,
MiravetVerde Samuel,
PiñeroLambea Carlos,
Serrano Luis,
LluchSenar Maria
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13714
Subject(s) - transposable element , biology , genome , transposon mutagenesis , genetics , mutagenesis , gene , computational biology , mutant
We utilised a novel genome deletion strategy involving the Cre/Lox system, coupled with random transposon mutagenesis, in the genome reduced bacterium Mycoplasma pneumoniae. We successfully demonstrated this approach can self‐selectively create a library of diverse and large genome deletions, and demonstrate a sequencing strategy capable of accurately identifying them.