Open AccessElevated c‐di‐GMP levels promote biofilm formation and biodesulfurization capacity of Rhodococcus erythropolis
Author(s) -
DoradoMorales Pedro,
Martínez Igor,
RiveroBuceta Virginia,
Díaz Eduardo,
Bähre Heike,
Lasa Iñigo,
Solano Cristina
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13689
Subject(s) - biofilm , rhodococcus , dibenzothiophene , bioremediation , chemistry , bioreactor , sulfur , bacteria , biochemistry , biology , organic chemistry , enzyme , genetics
In this work, we addressed the major hurdle of developing novel Rhodococcus erythropolis biocatalysts with improved biodesulfurization ability. To do so, we promoted Rhodococcus capability of forming a biofilm through the heterologous expression of a diguanylate cyclase that led to the synthesis of the biofilm trigger molecule cyclic‐di‐GMP (c‐di‐GMP). This approach resulted in an improved capacity to desulfurize dibenzothiophene and the ability to form a strong biofilm on surfaces under continuous flow conditions.