Open AccessImproved production of 2,3‐butanediol and isobutanol by engineering electron transport chain in Escherichia coli
Author(s) -
Jung HwiMin,
Han JaeHo,
Oh MinKyu
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13669
Subject(s) - isobutanol , metabolic engineering , oxidative phosphorylation , biochemistry , electron transport chain , nad+ kinase , escherichia coli , cofactor , yield (engineering) , chemistry , generally recognized as safe , adenosine triphosphate , heterologous , biology , ethanol , enzyme , materials science , metallurgy , gene
In this study, we attempted to reduce the electron transport chain (ETC) activity by making multiple mutants to secure availability of NADH, and then heterologous pathways for synthesis of 2,3‐butanediol and isobutanol were introduced. ETC mutants produced higher levels of metabolites with higher NADH/NAD + ratio. The results proved that engineering of ETC is a useful metabolic engineering approach for production of reduced metabolites.