Open AccessToxoplasma gondii α‐amylase deletion mutant is a promising vaccine against acute and chronic toxoplasmosis
Author(s) -
Yang Jing,
Yang Chenghang,
Qian Jiahui,
Li Facai,
Zhao Junlong,
Fang Rui
Publication year - 2020
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13668
Subject(s) - toxoplasmosis , toxoplasma gondii , biology , immunity , immune system , vaccination , immunology , virology , virulence , immunization , attenuated vaccine , microbiology and biotechnology , antigen , antibody , gene , genetics
In the study, we first generated a ME49Δ α‐amy mutant and discovered that loss of α‐AMY robustly grew in vitro but contributed to significant virulence attenuation in vivo . The results obtained from our study revealed that ME49Δ α‐amy attenuated strain could provide efficient protection against acute infection and chronic infection caused by multiple strains, which signified that ME49Δ α‐amy mutant might be a potential vaccine candidate.