Open AccessEfficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents
Author(s) -
Zou Gen,
Xiao Meili,
Chai Shunxing,
Zhu Zhihua,
Wang Ying,
Zhou Zhihua
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13652
Subject(s) - genome editing , biology , trans activating crrna , cas9 , crispr , genome , guide rna , ribonucleoprotein , rna , computational biology , genetics , gene
We report an optimized genome editing approach for filamentous fungi based on RNPs facilitated by adding chemical reagents. We increased the gene editing efficiency of RNPs significantly by adding Triton X‐100. Reagents related to mitosis and cell division furtherly increased ratio of edited homokaryotic transformants from filamentous fungus containing multinucleate spores and protoplasts.