Open AccessConsolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization
Author(s) -
Wen Zhiqiang,
Li Qi,
Liu Jinle,
Jin Mingjie,
Yang Sheng
Publication year - 2020
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13478
Subject(s) - clostridia , bioprocess , pentose , butanol , cellulosic ethanol , cellulase , fermentation , metabolic engineering , microbiology and biotechnology , chemistry , biology , cellulose , biochemistry , ethanol , enzyme , paleontology , genetics , bacteria
Summary Butanol is an important bulk chemical, as well as a promising renewable gasoline substitute, that is commonly produced by solventogenic Clostridia. The main cost of cellulosic butanol fermentation is caused by cellulases that are required to saccharify lignocellulose, since solventogenic Clostridia cannot efficiently secrete cellulases. However, cellulolytic Clostridia can natively degrade lignocellulose and produce ethanol, acetate, butyrate and even butanol. Therefore, cellulolytic Clostridia offer an alternative to develop consolidated bioprocessing ( CBP ), which combines cellulase production, lignocellulose hydrolysis and co‐fermentation of hexose/pentose into butanol in one step. This review focuses on CBP advances for butanol production of cellulolytic Clostridia and various synthetic biotechnologies that drive these advances. Moreover, the efforts to optimize the CBP ‐enabling cellulolytic Clostridia chassis are also discussed. These include the development of genetic tools, pentose metabolic engineering and the improvement of butanol tolerance. Designer cellulolytic Clostridia or consortium provide a promising approach and resource to accelerate future CBP for butanol production.