Open AccessA panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep
Author(s) -
Barral Thiago Doria,
Mariutti Ricardo Barros,
Arni Raghuvir Krishnaswamy,
Santos Anderson Jesus,
Loureiro Dan,
Sokolonski Ana Rita,
Azevedo Vasco,
Borsuk Sibele,
Meyer Roberto,
Portela Ricardo Dias
Publication year - 2019
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13454
Subject(s) - caseous lymphadenitis , corynebacterium pseudotuberculosis , recombinant dna , antigen , biology , antibody , microbiology and biotechnology , serology , immunology , bacteria , biochemistry , genetics , gene
Summary Caseous lymphadenitis ( CLA ) is a small ruminant disease characterized by the development of granulomatous lesions in superficial and internal lymph nodes, as well as in some organs, and causes significant economic losses worldwide. The aetiological agent of CLA is the bacterium Corynebacterium pseudotuberculosis ; however, the commercially available diagnostic tools present problems with regard to specificity, which can lead to false‐negative results. This study aimed to develop an indirect enzyme‐linked immunosorbent assay ( ELISA ) for the detection of specific immunoglobulins in goats and sheep using recombinant C. pseudotuberculosis PLD , CP 40, PknG, DtxR and Grx proteins. For validation of the ELISA s, 130 goat serum samples and 160 sheep serum samples were used. The best ELISA for goats was developed using a combination of PLD and CP 40 as antigens at a 1:1 ratio, which presented 96.9% sensitivity and 98.4% specificity. The most effective ELISA for sheep presented 91% sensitivity and 98.7% specificity when recombinant PLD alone was used as the antigen. These ELISA s can be used as highly accurate tools in epidemiological surveys and for the serodiagnosis of C. pseudotuberculosis infection in goats and sheep.