Open AccessDirect effects of organic pollutants on the growth and gene expression of the Baltic Sea model bacterium Rheinheimera sp. BAL 341
Author(s) -
Karlsson Christofer M. G.,
CerroGálvez Elena,
Lundin Daniel,
Karlsson Camilla,
VilaCosta Maria,
Pinhassi Jarone
Publication year - 2019
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13441
Subject(s) - bacterioplankton , bacteria , environmental chemistry , pollutant , bacterial growth , biology , metabolism , marine bacteriophage , microbial metabolism , heterotroph , food science , chemistry , microbiology and biotechnology , ecology , biochemistry , nutrient , phytoplankton , genetics
Summary Organic pollutants ( OP s) are critically toxic, bioaccumulative and globally widespread. Moreover, several OP s negatively influence aquatic wildlife. Although bacteria are major drivers of the ocean carbon cycle and the turnover of vital elements, there is limited knowledge of OP effects on heterotrophic bacterioplankton. We therefore investigated growth and gene expression responses of the Baltic Sea model bacterium Rheinheimera sp. BAL 341 to environmentally relevant concentrations of distinct classes of OP s in 2‐h incubation experiments. During exponential growth, exposure to a mix of polycyclic aromatic hydrocarbons, alkanes and organophosphate esters (denoted MIX ) resulted in a significant decrease (between 9% and 18%) in bacterial abundance and production compared with controls. In contrast, combined exposure to perfluorooctanesulfonic acids and perfluorooctanoic acids (denoted PFAS ) had no significant effect on growth. Nevertheless, MIX and PFAS exposures both induced significant shifts in gene expression profiles compared with controls in exponential growth. This involved several functional metabolism categories (e.g. stress response and fatty acids metabolism), some of which were pollutant‐specific (e.g. phosphate acquisition and alkane‐1 monooxygenase genes). In stationary phase, only two genes in the MIX treatment were significantly differentially expressed. The substantial direct influence of OP s on metabolism during bacterial growth suggests that widespread OP s could severely alter biogeochemical processes governed by bacterioplankton.