Open AccessLime and ammonium carbonate fumigation coupled with bio‐organic fertilizer application steered banana rhizosphere to assemble a unique microbiome against Panama disease
Author(s) -
Shen Zongzhuan,
Wang Beibei,
Zhu Jiaxin,
Hu Hangwei,
Tao Chengyuan,
Ou Yannan,
Deng Xuhui,
Ling Ning,
Li Rong,
Shen Qirong
Publication year - 2019
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13391
Subject(s) - rhizosphere , ammonium bicarbonate , biology , biofertilizer , fumigation , soil microbiology , fusarium oxysporum , agronomy , cuminum , botany , soil water , ecology , bacteria , essential oil , raw material , genetics
Summary Microbiome plays a key role in determining soil suppressiveness against invading pathogens. Our previous study revealed that microbial community of bulk soil could be manipulated by lime and ammonium bicarbonate fumigation followed by biofertilizer application. However, the assembly of microbial community suppressive to banana Panama disease in the rhizosphere is still unclear. In this study, we used high‐throughput sequencing and quantitative PCR to explore the assembly of rhizosphere microbiome associated with banana Panama disease suppression in a two‐seasonal pot experiment. We found biofertilizer applied to lime and ammonium bicarbonate fumigated soil significantly ( P < 0.05) reduced the abundance of rhizosphere Fusarium oxysporum compared to biofertilizer applied to non‐fumigated soil. Principal coordinate analysis revealed that biofertilizer applied to lime and ammonium bicarbonate fumigated soil re‐shaped the rhizosphere bacterial community composition by increasing the phylogenetic relatedness, and stimulating indigenous microbes, for example , Gemmatimonas , Sphingomonas , Pseudomonas , Lysobacter and Bacillus . Co‐occurrence analysis revealed that potential species involved in disease suppression were more interrelated in disease‐suppressive soils. Taken together, lime and ammonium bicarbonate fumigation followed by biofertilizer application could induce banana rhizosphere to assemble beneficial microbes dominated consortia to suppress banana Panama disease.