Open AccessGenome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID 13488
Author(s) -
HernándezArriaga Ana M.,
Cerro Carlos,
Urbina Leire,
Eceiza Arantxa,
Corcuera Mª Angeles,
Retegi Aloña,
Auxiliadora Prieto M.
Publication year - 2019
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13376
Subject(s) - operon , gene , genome , nanocellulose , bacterial cellulose , strain (injury) , biosynthesis , plasmid , cellulose , biology , bacteria , whole genome sequencing , chemistry , microbiology and biotechnology , biochemistry , genetics , escherichia coli , anatomy
Summary Komagataeibacter medellinensis ID 13488 (formerly Gluconacetobacter medellinensis ID 13488) is able to produce crystalline bacterial cellulose ( BC ) under high acidic growth conditions. These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production). To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb ( pKM 01), 4.3 kb ( pKM 02) and 3.3 Kb ( pKM 03). Genome comparison analyses of K. medellinensis ID 13488 with other cellulose‐producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis. Genes arrangement and composition of four bcs clusters ( bcs1, bcs2, bcs3 and bcs4 ) was studied by RT ‐ PCR , and their organization in four operons transcribed as four independent polycistronic mRNA s was determined. qRT ‐ PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC . Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.