Open AccessDiet and diet‐associated bacteria shape early microbiome development in Yellowtail Kingfish ( Seriola lalandi )
Author(s) -
Wilkes Walburn Jackson,
Wemheuer Bernd,
Thomas Torsten,
Copeland Elizabeth,
O'Connor Wayne,
Booth Mark,
Fielder Stewart,
Egan Suhelen
Publication year - 2019
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13323
Subject(s) - biology , microbiome , firmicutes , proteobacteria , zoology , larva , aquaculture , live food , ecology , 16s ribosomal rna , fishery , bacteria , fish <actinopterygii> , bioinformatics , genetics
Summary The supply of quality juveniles via land‐based larviculture represents a major bottleneck to the growing finfish aquaculture industry. As the microbiome plays a key role in animal health, this study aimed to assess the microbial community associated with early larval development of commercially raised Yellowtail Kingfish ( Seriola lalandi ). We used qPCR and 16S rRNA gene amplicon sequencing to monitor changes in the microbiome associated with the development of S. lalandi from larvae to juveniles. We observed an increase in the bacterial load during larval development, which consisted of a small but abundant core microbiota including taxa belonging to the families Rhodobacteraceae, Lactobacillaceae and Vibrionaceae . The greatest change in the microbiome occurred as larvae moved from a diet of live feeds to formulated pellets, characterized by a transition from Proteobacteria to Firmicutes as the dominant phylum. A prediction of bacterial gene functions found lipid metabolism and secondary metabolite production were abundant in the early larval stages, with carbohydrate and thiamine metabolism functions increasing in abundance as the larvae age and are fed formulated diets. Together, these results suggest that diet is a major contributor to the early microbiome development of commercially raised S. lalandi .