Open AccessStudies on the aerobic utilization of synthesis gas (syngas) by wild type and recombinant strains of Ralstonia eutropha H16
Author(s) -
Heinrich Daniel,
Raberg Matthias,
Steinbüchel Alexander
Publication year - 2018
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12873
Subject(s) - ralstonia , carbon monoxide dehydrogenase , recombinant dna , syngas , chemistry , gene , metabolic engineering , bacteria , strain (injury) , biochemistry , biology , carbon monoxide , microbiology and biotechnology , genetics , catalysis , anatomy
Summary The biotechnical platform strain Ralstonia eutropha H16 was genetically engineered to express a cox subcluster of the carboxydotrophic Oligotropha carboxidovorans OM 5 , including (i) the structural genes coxM , ‐ S and ‐ L , coding for an aerobic carbon monoxide dehydrogenase ( CODH ) and (ii) the genes cox D , ‐ E , ‐ F and ‐ G , essential for the maturation of CODH . The cox Oc genes expressed under control of the CO 2 ‐inducible promoter P L enabled R. eutropha to oxidize CO to CO 2 for the use as carbon source, as demonstrated by 13 CO experiments, but the recombinant strains remained dependent on H 2 as external energy supply. Therefore, a synthetic metabolism, which could be described as ‘carboxyhydrogenotrophic’, was established in R. eutropha . With this extension of the bacterium's substrate range, growth in CO ‐, H 2 ‐ and CO 2 ‐containing artificial synthesis gas atmosphere was enhanced, and poly(3‐hydroxybutyrate) synthesis was increased by more than 20%.