Open AccessProduction of a novel medium chain length poly(3‐hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold
Author(s) -
Basnett Pooja,
Lukasiewicz Barbara,
Marcello Elena,
Gura Harpreet K.,
Knowles Jonathan C.,
Roy Ipsita
Publication year - 2017
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12782
Subject(s) - biodiesel , polyhydroxyalkanoates , raw material , scaffold , tissue engineering , chemistry , transesterification , chemical engineering , biodiesel production , c2c12 , materials science , food science , pulp and paper industry , nuclear chemistry , organic chemistry , methanol , biomedical engineering , biochemistry , catalysis , biology , in vitro , engineering , myogenesis , bacteria , genetics
Summary This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocina CH 50, for the production of PHA s. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3‐hydroxyhexanoate‐ co ‐3‐hydroxyoctanoate‐ co‐ 3‐hydroxydecanoate‐ co ‐3‐hydroxydodecanoate) or P(3 HH x‐3 HO ‐3 HD ‐3 HDD ). P(3 HH x‐3 HO ‐3 HD ‐3 HDD ) was further characterized and evaluated for its use as a tissue engineering scaffold ( TES ). This study demonstrated that P(3 HH x‐3 HO ‐3 HD ‐3 HDD ) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3 HH x‐3 HO ‐3 HD ‐3 HDD ) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.