Open AccessAntimicrobial activity of biogenically produced spherical Se‐nanomaterials embedded in organic material against Pseudomonas aeruginosa and Staphylococcus aureus strains on hydroxyapatite‐coated surfaces
Author(s) -
Piacenza Elena,
Presentato Alessandro,
Zonaro Emanuele,
Lemire Joseph A.,
Demeter Marc,
Vallini Giovanni,
Turner Raymond J.,
Lampis Silvia
Publication year - 2017
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12700
Subject(s) - antimicrobial , staphylococcus aureus , biofilm , pseudomonas aeruginosa , microbiology and biotechnology , chemistry , bacteria , biology , genetics
Summary In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite ( HA )‐based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of S pherical biogenic Se‐ N anostructures Em bedded in O rganic material (Bio Se‐ NEMO ‐S) produced by Bacillus mycoides Sel TE 01 in comparison with two different chemical selenium nanoparticle (Se NP ) classes. These nanomaterials have been studied as potential antimicrobials for eradication of established HA ‐grown biofilms, for preventing biofilm formation on HA ‐coated surfaces and for inhibition of planktonic cell growth of Pseudomonas aeruginosa NCTC 12934 and Staphylococcus aureus ATCC 25923. Bio Se‐ NEMO resulted more efficacious than those chemically produced in all tested scenarios. Bio Se‐ NEMO produced by B. mycoides Sel TE 01 after 6 or 24 h of Na 2 SeO 3 exposure show the same effective antibiofilm activity towards both P. aeruginosa and S. aureus strains at 0.078 mg ml −1 (Bio Se‐ NEMO 6 ) and 0.3125 mg ml −1 (Bio Se‐ NEMO 24 ). Meanwhile, chemically synthesized Se NP s at the highest tested concentration (2.5 mg ml −1 ) have moderate antimicrobial activity. The confocal laser scanning micrographs demonstrate that the majority of the P. aeruginosa and S. aureus cells exposed to biogenic Se NP s within the biofilm are killed or eradicated. Bio Se‐ NEMO therefore displayed good antimicrobial activity towards HA ‐grown biofilms and planktonic cells, becoming possible candidates as new antimicrobials.