Open AccessRegulation of solvent tolerance in P seudomonas putida S12 mediated by mobile elements
Author(s) -
Hosseini Rohola,
Kuepper Jannis,
Koebbing Sebastian,
Blank Lars M.,
Wierckx Nick,
Winde Johannes H.
Publication year - 2017
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12495
Subject(s) - pseudomonas putida , mobile genetic elements , solvent , genome , adaptation (eye) , strain (injury) , bacteria , chemistry , biology , computational biology , biochemistry , genetics , gene , anatomy , neuroscience
Summary Organic solvent‐tolerant bacteria are outstanding and versatile hosts for the bio‐based production of a broad range of generally toxic aromatic compounds. The energetically costly solvent tolerance mechanisms are subject to multiple levels of regulation, involving among other mobile genetic elements. The genome of the solvent‐tolerant Pseudomonas putida S12 contains many such mobile elements that play a major role in the regulation and adaptation to various stress conditions, including the regulation of expression of the solvent efflux pump Srp ABC . We recently sequenced the genome of P. putida S12. Detailed annotation identified a threefold higher copy number of the mobile element ISS 12 in contrast to earlier observations. In this study, we describe the mobile genetic elements and elaborate on the role of ISS 12 in the establishment and maintenance of solvent tolerance in P. putida . We identified three different variants of ISS 12 of which a single variant exhibits a high translocation rate. One copy of this variant caused a loss of solvent tolerance in the sequenced strain by disruption of srpA . Solvent tolerance could be restored by applying selective pressure, leading to a clean excision of the mobile element.