Open AccessEnterobacter sp. LU 1 as a novel succinic acid producer – co‐utilization of glycerol and lactose
Author(s) -
Podleśny Marcin,
Jarocki Piotr,
Wyrostek Jakub,
Czernecki Tomasz,
Kucharska Jagoda,
Nowak Anna,
Targoński Zdzisław
Publication year - 2017
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12458
Subject(s) - succinic acid , enterobacter , lactose , fermentation , food science , glycerol , biochemistry , strain (injury) , chemistry , biology , 16s ribosomal rna , microbiology and biotechnology , escherichia coli , gene , anatomy
Summary Succinic acid is an important C4‐building chemical platform for many applications. A novel succinic acid‐producing bacterial strain was isolated from goat rumen. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the genus Enterobacter . This is the first report of a wild bacterial strain from the genus Enterobacter that is capable of efficient succinic acid production. Co‐fermentation of glycerol and lactose significantly improved glycerol utilization under anaerobic conditions, debottlenecking the utilization pathway of this valuable biodiesel waste product. Succinic acid production reached 35 g l −1 when Enterobacter sp. LU 1 was cultured in medium containing 50 g l −1 of glycerol and 25 g l −1 of lactose as carbon sources.