Open AccessEngineering Pseudomonas putida KT 2440 for simultaneous degradation of carbofuran and chlorpyrifos
Author(s) -
Gong Ting,
Liu Ruihua,
Che You,
Xu Xiaoqing,
Zhao Fengjie,
Yu Huilei,
Song Cunjiang,
Liu Yanping,
Yang Chao
Publication year - 2016
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12381
Subject(s) - carbofuran , pseudomonas putida , bioremediation , chlorpyrifos , recombinant dna , chemistry , strain (injury) , microbiology and biotechnology , kanamycin , pesticide , food science , bioaugmentation , biology , contamination , biochemistry , agronomy , enzyme , gene , ecology , anatomy
Summary Currently, chlorpyrifos ( CP ) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP /carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT 2440 using a chromosomal scarless modification strategy with upp as a counter‐selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for in situ bioremediation of soil co‐contaminated with carbofuran and CP .