Open AccessMultiple Locus Variable number of tandem repeat Analysis: A molecular genotyping tool for Paenibacillus larvae
Author(s) -
Descamps Tine,
De Smet Lina,
Stragier Pieter,
De Vos Paul,
Graaf Dirk C.
Publication year - 2016
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12375
Subject(s) - multiple loci vntr analysis , american foulbrood , genotyping , biology , variable number tandem repeat , tandem repeat , typing , genotype , genetics , intergenic region , locus (genetics) , microsatellite , minisatellite , computational biology , genome , allele , gene , bacteria
Summary American Foulbrood, caused by Paenibacillus larvae , is the most severe bacterial disease of honey bees ( Apis mellifera ). To perform genotyping of P. larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution. Here, we propose Multiple Locus Variable number of tandem repeat Analysis ( MLVA ). MLVA has been used for typing a collection of 209 P. larvae strains from which 23 different MLVA types could be identified. Moreover, the developed methodology not only permits the identification of the four Enterobacterial Repetitive Intergenic Consensus ( ERIC ) genotypes, but allows also a discriminatory subdivision of the most dominant ERIC type I and ERIC type II genotypes. A biogeographical study has been conducted showing a significant correlation between MLVA genotype and the geographical region where it was isolated.