Open AccessProduction of a single cyclic type of fructooligosaccharide structure by inulin‐degrading Paenibacillus sp. LX 16 newly isolated from Jerusalem artichoke root
Author(s) -
Yao Zhihua,
Guo Jiqiang,
Tang Wenzhu,
Sun Zhen,
Hou Yingmin,
Li Xianzhen
Publication year - 2016
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12358
Subject(s) - inulin , jerusalem artichoke , fructooligosaccharide , paenibacillus , biology , prebiotic , bacteria , mesophile , food science , botany , 16s ribosomal rna , genetics
Summary A novel inulin‐degrading bacterium was isolated from a soil sample collected on Jerusalem artichoke roots. It is a Gram‐positive, aerobic, motile and central endospore‐forming straight rod, and exhibits phenotypic properties being consistent with its classification in the genus Paenibacillus . The predominant cellular fatty acids were anteiso‐C15:0, C16:0 and anteiso‐C17:0. This strain represents a novel species of the genus Paenibacillus on the basis of phenotypic data together with phylogenetic analysis, and it is here designated as LX 16 and deposited in China centre for type collection, China (= CCTCC 2015256). Strain LX 16 could produce a cyclofructooligosaccharide fructanotransferase catalysing the formation of one type of fructooligosaccharide ( FOS ) from inulin. The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6. Such homology in inulin degradation products may be beneficial for the functional FOS production.