Open AccessComparative studies of the composition of bacterial microbiota associated with the ruminal content, ruminal epithelium and in the faeces of lactating dairy cows
Author(s) -
Liu Junhua,
Zhang Mengling,
Zhang Ruiyang,
Zhu Weiyun,
Mao Shengyong
Publication year - 2016
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12345
Subject(s) - verrucomicrobia , firmicutes , bacteroidetes , feces , biology , proteobacteria , unifrac , microbiology and biotechnology , composition (language) , zoology , food science , clostridium , actinobacteria , 16s ribosomal rna , bacteria , linguistics , philosophy , genetics
Summary The objective of this research was to compare the composition of bacterial microbiota associated with the ruminal content ( RC ), ruminal epithelium ( RE ) and faeces of Holstein dairy cows. The RC , RE and faecal samples were collected from six Holstein dairy cows when the animals were slaughtered. Community compositions of bacterial 16S rRNA genes from RC , RE and faeces were determined using a MiSeq sequencing platform with bacterial‐targeting universal primers 338F and 806R. UniFrac analysis revealed that the bacterial communities of RC , RE and faeces were clearly separated from each other. Statistically significant dissimilarities were observed between RC and faeces ( P = 0.002), between RC and RE ( P = 0.003), and between RE and faeces ( P = 0.001). A assignment of sequences to taxa showed that the abundance of the predominant phyla Bacteroidetes was lower in RE than in RC , while a significant higher ( P < 0.01) abundance of Proteobacteria was present in RE than in RC . When compared with the RC , the abundance of Firmicutes and Verrucomicrobia was higher in faeces, and RC contained a greater abundance of Bacteroidetes and Tenericutes. A higher proportions of Butyrivibrio and Campylobacter dominated RE as compared to RC . The faecal microbiota was less diverse than RC and dominated by genera Turicibacter and Clostridium . In general, these findings clearly demonstrated the striking compositional differences among RC , RE and faeces, indicating that bacterial communities are specific and adapted to the harbouring environment.