Open AccessThe acid adaptive tolerance response in C ampylobacter jejuni induces a global response, as suggested by proteomics and microarrays
Author(s) -
Varsaki Athanasia,
Murphy Caroline,
Barczynska Alicja,
Jordan Kieran,
Carroll Cyril
Publication year - 2015
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12302
Subject(s) - proteomics , gene , dna microarray , biology , adaptation (eye) , campylobacter jejuni , bacteria , gene expression , genetics , biochemistry , neuroscience
Summary C ampylobacter jejuni CI 120 is a natural isolate obtained during poultry processing and has the ability to induce an acid tolerance response ( ATR ) to acid + aerobic conditions in early stationary phase. Other strains tested they did not induce an ATR or they induced it in exponential phase. C ampylobacter spp. do not contain the genes that encode the global stationary phase stress response mechanism. Therefore, the aim of this study was to identify genes that are involved in the C . jejuni CI 120 early stationary phase ATR , as it seems to be expressing a novel mechanism of stress tolerance. Two‐dimensional gel electrophoresis was used to examine the expression profile of cytosolic proteins during the C . jejuni CI 120 adaptation to acid + aerobic stress and microarrays to determine the genes that participate in the ATR . The results indicate induction of a global response that activated a number of stress responses, including several genes encoding surface components and genes involved with iron uptake. The findings of this study provide new insights into stress tolerance of C . jejuni , contribute to a better knowledge of the physiology of this bacterium and highlight the diversity among different strains.