Open AccessCharacterization of microbial community structure during continuous anaerobic digestion of straw and cow manure
Author(s) -
Sun Li,
Pope Phillip B.,
Eijsink Vincent G. H.,
Schnürer Anna
Publication year - 2015
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12298
Subject(s) - methanosaeta , firmicutes , biology , bacteroidetes , clostridia , ruminococcus , manure , microbial population biology , species richness , bacteria , archaea , straw , anaerobic digestion , food science , methanosarcina , agronomy , ecology , 16s ribosomal rna , methane , genetics
Summary Responses of bacterial and archaeal communities to the addition of straw during anaerobic digestion of manure at different temperatures (37° C , 44° C and 52° C ) were investigated using five laboratory‐scale semi‐continuous stirred tank reactors. The results revealed that including straw as co‐substrate decreased the species richness for bacteria, whereas increasing the operating temperature decreased the species richness for both archaea and bacteria, and also the evenness of the bacteria. Taxonomic classifications of the archaeal community showed that M ethanobrevibacter dominated in the manure samples, while M ethanosarcina dominated in all digesters regardless of substrate. Increase of the operating temperature to 52° C led to increased relative abundance of M ethanoculleus and M ethanobacterium . Among the bacteria, the phyla F irmicutes and B acteroidetes dominated within all samples. Compared with manure itself, digestion of manure resulted in a higher abundance of an uncultured class WWE 1 and lower abundance of B acilli. Adding straw to the digesters increased the level of B acteroidia, while increasing the operating temperature decreased the level of this class and instead increased the relative abundance of an uncultured genus affiliated to order MBA 08 ( C lostridia). A considerable fraction of bacterial sequences could not be allocated to genus level, indicating that novel phylotypes are resident in these communities.