Open AccessProtein transduction domain of transactivating transcriptional activator fused to outer membrane protein K of V ibrio parahaemolyticus to vaccinate marbled eels ( A nguilla marmorata ) confers protection against mortality caused by V . parahaemolyticus
Author(s) -
Wang Hang,
Yang Wei,
Shen Guoying,
Zhang Jianting,
Lv Wei,
Ji Binfeng,
Meng Chun
Publication year - 2015
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12281
Subject(s) - transduction (biophysics) , immune system , biology , vaccination , antibody , microbiology and biotechnology , activator (genetics) , downregulation and upregulation , chemistry , virology , gene , immunology , biochemistry
Summary Although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. We used the protein transduction domain ( PTD ) of transactivating transcriptional factor ( TAT ) derived from HIV TAT protein to increase the delivery efficiency of aquatic protein vaccines. V ibrio parahaemolyticus outer membrane protein K ( ompK ), a reported vaccine candidate for the prevention of V . parahaemolyticus infection, was fused with TAT ‐ PTD expressed in E scherichia coli . We found that PTD ‐ ompK fusion protein effectively penetrated into marbled eel bodies. Analysis of ompK antibody titres demonstrated that immersion vaccination with PTD ‐ ompK was superior to ompK alone and induced robust immune stimulation in marbled eels. Both active and passive protection analyses against immersive challenge with V . parahaemolyticus strains demonstrated that marbled eels immunized with PTD ‐ ompK survived significantly longer than those immunized with ompK alone. Our results indicated that TAT ‐ PTD could be served as is an efficient delivery system for aquatic immersion vaccinations against various infectious diseases commonly seen in aquatic farm industry.