Open AccessGenetic characterization of caffeine degradation by bacteria and its potential applications
Author(s) -
Summers Ryan M.,
Mohanty Sujit K.,
Gopishetty Sridhar,
Subramanian Mani
Publication year - 2015
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12262
Subject(s) - gene , biochemistry , bacteria , biology , demethylation , caffeine , dna , genomic dna , chemistry , genetics , dna methylation , gene expression , endocrinology
Summary The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N ‐demethylation and C ‐8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N ‐demethylation and C ‐8 oxidation. All of the genes for the N ‐demethylation pathway, encoding enzymes in the R ieske oxygenase family, reside on 13.2‐kb genomic DNA fragment found in P seudomonas putida CBB 5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in P seudomonas sp. CES . Similarly, genes for C ‐8 oxidation of caffeine have been located on a 25.2‐kb genomic DNA fragment of P seudomonas sp. CBB 1. The C ‐8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of K lebsiella pneumoniae . Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio‐decaffeination, remediation of caffeine‐contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated.