Open AccessEvaluation of industrial S accharomyces cerevisiae strains as the chassis cell for second‐generation bioethanol production
Author(s) -
Li Hongxing,
Wu Meiling,
Xu Lili,
Hou Jin,
Guo Ting,
Bao Xiaoming,
Shen Yu
Publication year - 2015
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12245
Subject(s) - xylose , hydrolysate , fermentation , saccharomyces cerevisiae , ethanol fuel , strain (injury) , food science , chassis , biofuel , yeast , chemistry , biochemistry , lignocellulosic biomass , ethanol , microbiology and biotechnology , biology , hydrolysis , structural engineering , anatomy , engineering
Summary To develop a suitable S accharomyces cerevisiae industrial strain as a chassis cell for ethanol production using lignocellulosic materials, 32 wild‐type strains were evaluated for their glucose fermenting ability, their tolerance to the stresses they might encounter in lignocellulosic hydrolysate fermentation and their genetic background for pentose metabolism. The strain BSIF , isolated from tropical fruit in T hailand, was selected out of the distinctly different strains studied for its promising characteristics. The maximal specific growth rate of BSIF was as high as 0.65 h −1 in yeast extract peptone dextrose medium, and the ethanol yield was 0.45 g g −1 consumed glucose. Furthermore, compared with other strains, this strain exhibited superior tolerance to high temperature, hyperosmotic stress and oxidative stress; better growth performance in lignocellulosic hydrolysate; and better xylose utilization capacity when an initial xylose metabolic pathway was introduced. All of these results indicate that this strain is an excellent chassis strain for lignocellulosic ethanol production.