Open AccessColonization by endophytic O chrobactrum anthropi M n1 promotes growth of J erusalem artichoke
Author(s) -
Meng Xianfa,
Yan Dekai,
Long Xiaohua,
Wang Changhai,
Liu Zhaopu,
Rengel Zed
Publication year - 2014
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12145
Subject(s) - nitrogen fixation , colonization , biology , jerusalem artichoke , quorum sensing , strain (injury) , bacteria , microbiology and biotechnology , 16s ribosomal rna , botany , genetics , anatomy , biofilm
Summary The O chrobactrum anthropi Mn 1 strain, taxonomically identified using 16S ribosomal DNA sequence, was isolated from roots of J erusalem artichoke. Its endophytic colonization was investigated microscopically using green fluorescent protein introduced by vector pHC 60. The strain entered Jerusalem artichoke tissues through the root, and was localized in the roots and stems. The plant growth‐promoting ( PGP ) effects of O . anthropi Mn 1 were assessed in greenhouse as well as field trials with different nitrogen supplies. Only under moderate to ample nitrogen supply, could O . anthropi Mn 1 promoted growth of host plant. The PGP effects of the strain were symbiotic nitrogen fixation, root morphological optimization and enhanced nutrient uptake. We hypothesize that the symbiotic interspecies interaction might be quorum sensing related.