Open AccessThe relationship between lysine 4 on histone H 3 methylation levels of alcohol tolerance genes and changes of ethanol tolerance in S accharomyces cerevisiae
Author(s) -
Wang Hang,
Ji Binfeng,
Ren Hongzhen,
Meng Chun
Publication year - 2014
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12121
Subject(s) - saccharomyces cerevisiae , methylation , epigenetics , yeast , ethanol , mutant , alcohol tolerance , biology , genetics , histone methylation , gene , lysine , biochemistry , dna methylation , gene expression , amino acid
Summary We evaluated whether epigenetic changes contributed to improve ethanol tolerance in mutant populations of S accharomyces cerevisiae ( S . cerevisiae ). Two ethanol‐tolerant variants of S . cerevisiae were used to evaluate the genetic stability in the process of stress‐free passage cultures. We found that acquired ethanol tolerance was lost and transcription level of some genes ( HSP 104 , PRO 1 , TPS 1 , and SOD 1 ) closely related to ethanol tolerance decreased significantly after the 10th passage in ethanol‐free medium. Tri‐methylation of lysine 4 on histone H 3 ( H 3 K 4) enhanced at the promoter of HSP 104 , PRO 1 , TPS 1 and SOD 1 in ethanol‐tolerant variants of S . cerevisiae was also diminished after tenth passage in stress‐free cultures. The ethanol tolerance was reacquired when exogenous SOD 1 transferred in some tolerance‐lost strains. This showed that H 3 K 4 methylation is involved in phenotypic variation with regard to ethanol tolerance with respect to classic breeding methods used in yeast.