Open AccessInitiation of the flexirubin biosynthesis in C hitinophaga pinensis
Author(s) -
Schöner Tim A.,
Fuchs Sebastian W.,
Schönau Christian,
Bode Helge B.
Publication year - 2014
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12110
Subject(s) - biosynthesis , biochemistry , gene cluster , polyene , chemistry , bacteria , thioester , tandem mass spectrometry , pigment , mass spectrometry , biology , gene , chromatography , organic chemistry , enzyme , genetics
Summary Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non‐isoprenoid aryl‐polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from C hitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia‐lyase and a 4‐coumarate‐ CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C . pinensis was characterized by a combination of feeding experiments, high‐performance liquid chromatography tandem mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry.