Open AccessXylose‐fermenting P ichia stipitis by genome shuffling for improved ethanol production
Author(s) -
Shi Jun,
Zhang Min,
Zhang Libin,
Wang Pin,
Jiang Li,
Deng Huiping
Publication year - 2014
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12092
Subject(s) - pichia stipitis , xylose , fermentation , xylose metabolism , yeast , bioconversion , dna shuffling , ethanol fuel , chemistry , biochemistry , food science , pentose , biology , directed evolution , mutant , gene
Summary Xylose fermentation is necessary for the bioconversion of lignocellulose to ethanol as fuel, but wild‐type S accharomyces cerevisiae strains cannot fully metabolize xylose. Several efforts have been made to obtain microbial strains with enhanced xylose fermentation. However, xylose fermentation remains a serious challenge because of the complexity of lignocellulosic biomass hydrolysates. Genome shuffling has been widely used for the rapid improvement of industrially important microbial strains. After two rounds of genome shuffling, a genetically stable, high‐ethanol‐producing strain was obtained. Designated as TJ 2‐3, this strain could ferment xylose and produce 1.5 times more ethanol than wild‐type P ichia stipitis after fermentation for 96 h. The acridine orange and propidium iodide uptake assays showed that the maintenance of yeast cell membrane integrity is important for ethanol fermentation. This study highlights the importance of genome shuffling in P . stipitis as an effective method for enhancing the productivity of industrial strains.