Open AccessMetabolic potential of the organic‐solvent tolerant P seudomonas putida DOT ‐ T1E deduced from its annotated genome
Author(s) -
Udaondo Zulema,
Molina Lazaro,
Daniels Craig,
Gómez Manuel J.,
MolinaHenares María A.,
Matilla Miguel A.,
Roca Amalia,
Fernández Matilde,
Duque Estrella,
Segura Ana,
Ramos Juan Luis
Publication year - 2013
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12061
Subject(s) - pseudomonas putida , operon , genome , metabolic engineering , transformation (genetics) , enzyme , strain (injury) , whole genome sequencing , biotransformation , computational biology , biology , gene , metabolic pathway , genetics , biochemistry , chemistry , escherichia coli , anatomy
Summary P seudomonas putida DOT ‐ T1E is an organic solvent tolerant strain capable of degrading aromatic hydrocarbons. Here we report the DOT ‐ T1E genomic sequence (6 394 153 bp) and its metabolic atlas based on the classification of enzyme activities. The genome encodes for at least 1751 enzymatic reactions that account for the known pattern of C , N , P and S utilization by this strain. Based on the potential of this strain to thrive in the presence of organic solvents and the subclasses of enzymes encoded in the genome, its metabolic map can be drawn and a number of potential biotransformation reactions can be deduced. This information may prove useful for adapting desired reactions to create value‐added products. This bioengineering potential may be realized via direct transformation of substrates, or may require genetic engineering to block an existing pathway, or to re‐organize operons and genes, as well as possibly requiring the recruitment of enzymes from other sources to achieve the desired transformation.