Open AccessSensitivity and specificity of PS / AA ‐modified nanoparticles used in malaria detection
Author(s) -
Thiramanas Raweewan,
Jangpatarapongsa Kulachart,
Asawapirom Udom,
Tangboriboonrat Pramuan,
Polpanich Duangporn
Publication year - 2013
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12021
Subject(s) - miniemulsion , chemistry , polyclonal antibodies , conjugated system , streptavidin , conjugate , agglutination (biology) , bioconjugation , polymerization , acrylic acid , biotin , monomer , combinatorial chemistry , antibody , biochemistry , polymer , organic chemistry , biology , immunology , mathematical analysis , mathematics
Summary Polystyrene ( PS ) nanoparticle ( NP ) copolymerized with acrylic acid ( AA ) and coloured monomer, i.e. 2,3,6,7‐tetra(2,2′‐bithiophene)‐1,4,5,8‐naphthalenetetracarboxylic‐ N , N ′ ‐di(2‐methylallyl)‐bisimide ( ALN8T ), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody ( Ab ) specific to P lasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin ( SA ) and biotin interaction, were employed. The optimum ratio of Ab to NP s used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NP s and malaria patient plasma were investigated. All Ab –latex conjugates provided the high sensitivity for the detection of P . falciparum malaria plasma. The highest specificity to P . falciparum was obtained from using Ab – NP s conjugated via the SA –biotin interaction.