Open AccessTranscriptional response of lignin‐degrading enzymes to 17α‐ethinyloestradiol in two white rots
Author(s) -
Přenosilová L.,
Křesinová Z.,
Amemori A. Slavíková,
Cajthaml T.,
Svobodová K.
Publication year - 2013
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12007
Subject(s) - laccase , bioremediation , manganese peroxidase , trametes versicolor , mycelium , enzyme , peroxidase , chemistry , lignin , biochemistry , biology , microbiology and biotechnology , food science , botany , bacteria , genetics
Summary Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, a transcriptional analyses of laccase and manganese peroxidase ( MnP ) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17α‐ethinyloestradiol ( EE 2) degradation to study regulation mechanisms used by fungi during EE 2 degradation. In the cultures of T rametes versicolor the addition of EE 2 caused an increase in laccase activity with a maximum of 34.2 ± 6.7 U g −1 of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9 times higher transcription levels of a laccase‐encoding gene ( lacB ) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE 2 treatment. Like T . versicolor , I rpex lacteus was also able to remove 10 mg l −1 EE 2 within 3 days of cultivation. While an increase to I . lacteus MnP activity and MnP gene transcription levels was observed at the later phase of the cultivation. It suggests another metabolic role of MnP but EE 2 degradation.