Open AccessAccelerated pentose utilization by C orynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine
Author(s) -
Meiswinkel Tobias M.,
Gopinath Vipin,
Lindner Steffen N.,
Nampoothiri K. Madhavan,
Wendisch Volker F.
Publication year - 2013
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.12001
Subject(s) - xylose , biochemistry , xylose isomerase , corynebacterium glutamicum , chemistry , pentose , arabinose , xylose metabolism , ornithine , fermentation , biology , arginine , amino acid , gene
Summary Because of their abundance in hemicellulosic wastes arabinose and xylose are an interesting source of carbon for biotechnological production processes. Previous studies have engineered several C orynebacterium glutamicum strains for the utilization of arabinose and xylose, however, with inefficient xylose utilization capabilities. To improve xylose utilization, different xylose isomerase genes were tested in C . glutamicum . The gene originating from X anthomonas campestris was shown to have the highest effect, resulting in growth rates of 0.14 h −1 , followed by genes from B acillus subtilis , M ycobacterium smegmatis and E scherichia coli . To further increase xylose utilization different xylulokinase genes were expressed combined with X . campestris xylose isomerase gene. All combinations further increased growth rates of the recombinant strains up to 0.20 h −1 and moreover increased biomass yields. The gene combination of X . campestris xylose isomerase and C . glutamicum xylulokinase was the fastest growing on xylose and compared with the previously described strain solely expressing E . coli xylose isomerase gene delivered a doubled growth rate. Productivity of the amino acids glutamate, lysine and ornithine, as well as the diamine putrescine was increased as well as final titres except for lysine where titres remained unchanged. Also productivity in medium containing rice straw hydrolysate as carbon source was increased.