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Correlation of diet, microbiota and metabolite networks in inflammatory bowel disease
Author(s) -
Weng Yi Jie,
Gan Huo Ye,
Li Xiang,
Huang Yun,
Li Zheng Chao,
Deng Hui Min,
Chen Su Zuan,
Zhou Yu,
Wang Li Sheng,
Han Yan Ping,
Tan Ya Fang,
Song Ya Jun,
Du Zong Min,
Liu Yang Yang,
Wang Ye,
Qin Nan,
Bai Yang,
Yang Rui Fu,
Bi Yu Jing,
Zhi Fa Chao
Publication year - 2019
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12795
Subject(s) - gut flora , fusobacterium , inflammatory bowel disease , dysbiosis , bacteroides fragilis , metabolite , microbiology and biotechnology , bacteroides , feces , fusobacteria , medicine , microbiome , biology , physiology , firmicutes , biochemistry , disease , antibiotics , bacteria , 16s ribosomal rna , bioinformatics , genetics , gene
Objectives Microbiota dysbiosis in inflammatory bowel disease (IBD) has been widely reported. The gut microbiota connect diet to the metabolism by producing small molecules via diverse metabolic pathways. In this study we aimed to investigate the dietary preferences of IBD patients, and to explore the interactions among gut microbiota composition, dietary components, and metabolites in relation to IBD. Methods Dietary preferences of IBD patients (including those with ulcerative colitis [UC] and Crohn's disease [CD]) and health controls were investigated, and their gut microbiota were analyzed using 16S rRNA gene sequencing and metagenomic analyses of fecal and biopsy samples. The metabolite profiles of the samples were then analyzed using gas and liquid chromatography‐mass spectrometry analyses. Results The daily intake of folic acid, niacin, vitamins C and D, calcium, and selenium differed significantly between patients with IBD and healthy controls. A decrease in long‐chain (such as arachidic, and oleic acid) and medium‐chain fatty acids (sebacic acid and isocaproic acid) as well as bile acid was observed in patients with IBD. Compared with healthy controls, 22 microbial species (including Sulfolobus acidocaldarius , and Clostridium clostridioforme CAG132 ) in the UC group and 37 microbial species (such as Bacteroides fragilis and Fusobacterium nucleatum ) in the CD group were found to be correlated to diet and metabolites. Bacteroides fragilis was enriched in patients with IBD and associated with multi‐nutrients, and 21 metabolites including 25‐hydroxyvitamin D 3 and taurolithocholic acid. Conclusions This study provides an interaction network to identify key micronutrients, microbiota components and metabolites that contribute to IBD.