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Evaluation of three techniques for detection of IL28B SNP: A prognostic tool for HCV treatment outcome
Author(s) -
Khubaib Bushra,
Idrees Muhammad,
Fatima Zareen,
Akram Madiha,
Afzal Samia,
Amin Iram,
Shahid Muhammad,
Wasim Muhammad
Publication year - 2017
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12497
Subject(s) - genotyping , restriction fragment length polymorphism , single nucleotide polymorphism , medicine , snp , polymerase chain reaction , genotype , snapshot (computer storage) , computational biology , biology , genetics , gene , computer science , database
OBJECTIVE The study was aimed to evaluate the specificity, cost and turnaround time of three different techniques that can be used for analyzing the single nucleotide polymorphism of interleukin 28B ( IL28B ) rs129796860. METHODS DNA from peripheral blood samples of 111 patients with chronic hepatitis C were genotyped using three types of genotyping methods: direct sequencing, SNaPshot polymerase chain reaction (PCR) and PCR‐restriction fragment length polymorphism (PCR‐RFLP). RESULTS Three distinct profiles for IL28B rs12979860 alleles (CC, CT and TT) were obtained with direct sequencing, SNaPshot PCR and PCR‐RFLP and the results were consistent among all three methods. CONCLUSION For routine medical practice, screening IL28B rs12979860 can be performed by PCR‐RFLP, which is efficient and reliable as well as cost‐effective.