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Micro RNA profile analysis in the liver fibrotic tissues of chronic hepatitis B patients
Author(s) -
Chen Rong,
Wu Jun Cheng,
Liu Ting,
Qu Ying,
Lu Lun Gen,
Xu Ming Yi
Publication year - 2017
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12452
Subject(s) - rna , fibrosis , medicine , receiver operating characteristic , chronic hepatitis , microarray , microrna , pathology , gene , gene expression , virus , biology , immunology , genetics
OBJECTIVE We aimed to identify the features of micro RNA (mi RNA ) at different fibrotic stages in patients with hepatitis B virus ( HBV )‐related liver fibrosis.METHODS Liver tissues were collected from 40 chronic hepatitis B ( CHB ) patients at fibrotic stages S 0–4. Microarrays of mi RNA s and genomic informatics analysis were performed.RESULTS In total, 105 mi RNA s were differentially expressed in fibrotic tissues ( S 1–4 groups) compared with no fibrotic tissues ( S 0 group; P < 0.05). Combined with three classifications, 17 differential mi RNA s were found to be closely related to fibrotic stages (over twofold change and P < 0.05). Five mi RNA s had a signature that correlated with serum biochemical parameters and liver inflammatory grades. The receiver operating characteristic ( ROC ) curve showed that six mi RNA s performed excellently in the diagnosis of liver fibrosis, with the area under the ROC curve (AUROC) over 0.8; among them hsa‐mi R ‐214‐3p had the highest AUROC (0.867). Gene ontology functions of differential mi RNA s mainly involved in the cellular and developmental processes, localization, biological regulation, binding, transcriptional regulator and organelle. We also found that 23 novel signaling pathways were dysregulated in the liver fibrosis.CONCLUSIONS Mi RNA profile signature, including 17 differential mi RNA s and 23 dysregulated signaling pathways, was associated with liver fibrosis. Hepatic inflammatory grades were correlated with the differential mi RNA . Some mi RNA s can be used for the diagnosis of liver fibrosis.