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Correlation between circulating miR‐122 and prognosis of chronic HBV‐related liver failure
Author(s) -
Wang Wei Jing,
Lai Rong Tao,
Lu Jie,
Xiang Xiao Gang,
Zhao Gang De,
Tang Wei Liang,
Cai Wei,
Wang Hui,
Zhou Hui Juan,
Xie Qing
Publication year - 2016
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12348
Subject(s) - medicine , cirrhosis , mir 122 , gastroenterology , biomarker , stage (stratigraphy) , liver disease , prothrombin time , liver injury , real time polymerase chain reaction , immunology , hepatitis c virus , virus , gene , biology , paleontology , biochemistry
Objective To assess the correlation between circulating microRNA (miR)‐122 level and the prognosis of chronic hepatitis B‐related liver failure (CHBLF). Methods Serum miR‐122 from CHBLF patients ( n = 6) and healthy controls ( n = 6) was quantified using an Exiqon locked nucleic acid microarray. Quantitative real‐time polymerase chain reaction was utilized to determine serum miR‐122 expression in 102 patients with different liver diseases [CHBLF ( n = 58), acute hepatitis B ( n = 10), chronic hepatitis B ( n = 22) and hepatitis B‐related cirrhosis ( n = 12)] and 23 healthy controls. The correlations between miR‐122 and disease stages based on prothrombin activity (PTA) and model for end‐stage liver disease (MELD) scores were further analyzed. Results Microarray showed that miR‐122 was significantly upregulated among 148 significantly modified miRNAs in CHBLF patients. Serum level of miR‐122 in CHBLF patients was significantly upregulated at early stage based on PTA or stages I–II based on MELD score. Interestingly, there was a significant correlation between the MELD score and circulating miR‐122 level in patients with an MELD score of <30 ( r = 0.521, P = 0.001). Moreover, serum level of miR‐122 was significantly decreased at discharge compared with that at admission as shown in the same group of CHBLF patients ( P < 0.05). Conclusions Serum level of miR‐122 is correlated with the severity of liver injury at an early stage. miR‐122 may be a potential biomarker for both the diagnosis at an early stage of CHBLF and the prognosis for recovery.