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The influence of H elicobacter pylori eradication on the expression and methylation status of the FHIT gene in non‐cancerous gastric mucosa of dyspeptic patients
Author(s) -
Peczek Lukasz,
Zuk Karolina,
StecMichalska Krystyna,
Medrek Marta,
Nawrot Barbara
Publication year - 2015
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12252
Subject(s) - fhit , helicobacter pylori , methylation , medicine , gastritis , gastric mucosa , biopsy , gastroenterology , cancer , dna methylation , real time polymerase chain reaction , gene expression , gene , biology , stomach , carcinogenesis , tumor suppressor gene , genetics
Objective To investigate the effect of H elicobacter pylori ( H. pylori ) eradication on the expression level of the FHIT gene and its methylation status in the gastric mucosa of dyspeptic patients with or without a family history of gastric cancer ( FHGC ). Methods In all, 31 patients with H. pylori infection including 13 with FHGC were enrolled in the study. The effectiveness of H. pylori eradication were confirmed by UBT , RUT and multiplex PCR (the presence of selected H . pylori strains) for biopsy samples from the antrum and corpus. Histopathological assessment was also performed. The expression of FHIT mRNA was determined by quantitative reverse transcription‐polymerase chain reaction and the methylation status of the FHIT promoter was assessed by methylation‐specific polymerase chain reaction. Results After H . pylori eradication, the improvement of inflammation from superficial gastritis to normal mucosa ( G → N ) was observed in 39% of the patients without FHGC and in 54% of those with FHGC . FHIT mRNA expression was increased in patients without FHGC after H. pylori eradication ( P < 0.05), while there was no statistically significant change in gene methylation status after H. pylori eradication ( P > 0.05). For the samples from those with FHGC , the FHIT mRNA expression was not significantly changed and the methylation status fluctuated evenly. Conclusions H. pylori eradication results in the improvement of gastric mucosal inflammation and histopathological non‐atrophic changes. The FHIT gene expression is increased in patients without FHGC , which may contribute to the prevention of GC development.