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The therapeutic role of oral tolerance in dextran sulfate sodium‐induced colitis via Th1–T h2 balance and γδ T cells
Author(s) -
Yue Min,
Shen Zhe,
Yu Chao Hui,
Ye Hua,
Li You Ming
Publication year - 2013
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12068
Subject(s) - colitis , medicine , intraepithelial lymphocyte , lamina propria , ovalbumin , inflammatory bowel disease , immunology , spleen , gastroenterology , pharmacology , pathology , immune system , disease , epithelium
Objective To evaluate the state of oral tolerance and its therapeutic role in mice with dextran sulfate sodium ( DSS )‐induced colitis. Methods Delayed‐type hypersensitivity ( DTH ) was determined 7 and 14 days after DSS ‐induced colitis and control mice. Disease activity index ( DAI ) score and colonic histopathological score were measured 7 days after colonic extracted protein ( CEP ) or bovine serum albumin ( BSA) (control ) was administrated, with the evaluation of Th1–T h2 balance in the spleen, P eyer's patch and γδ T cells in intraepithelial lymphocytes and lamina proper lymphocytes in the intestine. Results After fed with 250 μg ovalbumin oral tolerance was induced in 7 days in both DSS‐induced colitis and control mice, while oral tolerance persisted in the control mice but vanished in DSS ‐induced colitis 14 days after ovalbumin challenge. DAI and colonic histopathological scores were decreased significantly after the ingestion of CEP (controlled by BSA ) in DSS ‐induced colitis with significant reduction of T h1 and the ratio of Th1 to T h2 in P eyer's patch as well as the γδ T cells in lamina proper lymphocytes in the intestine. No significant difference in Th1–T h2 balance in the spleen and γδ T cells in intraepithelial lymphocytes in the intestine were observed. Conclusions There is a defect in oral tolerance at day 7 in DSS ‐induced colitis. If taken orally, CEP may have a protective role in DSS ‐induced colitis, which may be related to the deflection from T h1 to T h2 in P eyer's patch and the reduction of γδ T cells in lamina proper lymphocytes in the intestine.