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Effect of recombinant L actobacillus casei expressing interleukin‐10 in dextran sulfate sodium‐induced colitis mice
Author(s) -
Qiu Zhi Bing,
Chen Jian,
Chen Jia Jie,
Rong Lan,
Ding Wei Qun,
Yang Hai Jing,
Zhong Liang
Publication year - 2013
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/1751-2980.12006
Subject(s) - lactobacillus casei , recombinant dna , colitis , western blot , medicine , pharmacology , interleukin , microbiology and biotechnology , chemistry , immunology , biology , cytokine , biochemistry , fermentation , gene
Objective To study the effect of recombinant L actobacillus casei ( L.casei ) expressing interleukin ( IL )‐10 combined with 5‐aminosalicylic acid (5‐ ASA ) in dextran sulfate sodium ( DSS )‐induced colitis mice. Methods Recombinant L . casei CECT 5276, which can secrete IL ‐10, was constructed. The length of colon tissue, disease activity index ( DAI ) and histological score ( HS ) of the mice were determined to evaluate the modeling and the effectiveness of L . casei . Real‐time polymerase chain reaction ( PCR ), Western blot and ELISA were used to determine the levels of nuclear factor kappa‐light‐chain‐enhancer of activated B cells ( NF ‐κ B ), peroxisome proliferator‐activated receptor ( PPAR )‐γ, interferon ( IFN )‐γ, transforming growth factor ( TGF )‐β and IL ‐10. Results Recombinant L . casei expressing IL ‐10 combined with 5‐ ASA was more effective than L . casei with 5‐ ASA . Among the three different concentrations of the recombinant L . casei , the highest concentration group (2 × 10 9 colony‐forming units/m L ) had the best effectiveness. Conclusions Recombinant L . casei combined with 5‐ ASA is effective in the treatment of DSS ‐induced colitis. The possible mechanism might be the blocking of the excessive activation of NF ‐κ B pathway, thus suppressing the release of inflammation‐related factors.