Premium
Oral administration of Eucommia ulmoides Oliv . leaves extract protects against atherosclerosis by improving macrophage function in ApoE knockout mice
Author(s) -
HashikawaHobara Narumi,
Hashikawa Naoya,
Sugiman Nao,
Hosoo Shingo,
Hirata Tetsuya,
Yamaguchi Yasuyo,
Yamasaki Hiroo,
Kawasaki Hiromu,
Nishibe Sansei
Publication year - 2020
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.15461
Subject(s) - eucommia ulmoides , macrophage , apolipoprotein e , knockout mouse , medicine , inflammation , cytokine , apolipoprotein b , pharmacology , immunology , endocrinology , traditional medicine , traditional chinese medicine , chemistry , biochemistry , pathology , receptor , in vitro , cholesterol , alternative medicine , disease
Eucommia leaf extract (ELE) is a traditional Chinese herbal medicine. We investigated the effect of ELE on the development of atherosclerosis and changes in peritoneal macrophage function in apolipoprotein E knockout (ApoE –/– ) mice. At 8 weeks of age, ApoE –/– mice were randomly divided into three groups that were fed a high‐fat diet blended with 0% (control), 5% or 10% ELE for a period of 7 weeks. The 10% ELE dose caused an approximately 36% reduction in atherosclerotic lesions, as estimated by oil red O staining. Real‐time PCR analysis showed that the 1‐week treatment with ELE reduced mRNA levels of Tnf‐alpha , Il‐1 , and Mif in peritoneal macrophages isolated from the ApoE –/– mice. Furthermore, a 1‐week treatment with the 10% ELE diet significantly reduced migration and adhesion functions in peritoneal macrophages. These results suggest that a 10% ELE diet reduces atherosclerotic lesions and modulates macrophage function by reducing cytokine expression. Practical Application Eucommia leaf extract (ELE) is a traditional Chinese herbal medicine that reduces atherosclerotic lesions and suppresses inflammatory cytokines expression.