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Effects of soybean isoflavone on metabolism of rat osteoblasts and cytokines in vitro
Author(s) -
Lu Minan,
Xie Kegong,
Huang Ke,
Lu Xianzhe,
Lu Lu,
Shi Yu,
Tang Yujin
Publication year - 2020
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/1750-3841.14986
Subject(s) - alkaline phosphatase , medicine , endocrinology , osteoprotegerin , vascular endothelial growth factor , in vitro , osteoblast , transforming growth factor , chemistry , interleukin , biology , cytokine , biochemistry , enzyme , vegf receptors , receptor , activator (genetics)
The effects and mechanisms of soybean isoflavone on osteoblast (OB) proliferation in vitro were investigated. Fifty female Wistar rats were randomly divided into five groups with 10 rats in each group. Rat OBs were separated and cultured. The first generation of OBs cultured for 48 hr at various concentrations of isoflavone were set as the experimental groups, the OBs exposed to estradiol (E 2 ) culture were considered as positive control group. The biological characterization of OBs was investigated by phase contrast microscopy and alkaline phosphatase (ALP) histochemistry. The concentrations of interleukin (IL‐1), osteoprotegerin (OPG), transforming growth factor (TGF), granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), and vascular endothelial growth factor (VEGF) in isoflavone culture solutions were determined. Proliferation rate of OBs was increased in experimental group comparing that in the blank group. ALP activity in experimental group was higher than that in blank group. No significant differences of ALP activity were observed between E 2 culture group and isoflavone group at concentrations of 10 −5 and 10 −7 mM ( P > 0.05). Furthermore, in the experimental groups at low isoflavone concentrations, the concentrations of OPG, TGF, and VEGF were increased and positively correlated with OB proliferation. However, the concentrations of IL‐1, GM‐CSF were decreased at higher concentration of isoflavone and were negatively correlated with OB proliferation. Soybean isoflavone could promote the growth and proliferation of rat OB, it might act as the stimulator of OPG, TGF, and VEGF pathway, and the inhibitor of IL‐1, GM‐CSF pathway as well.